1. Neumann T., Bonham A. J., Dame G., Bertold B., Brandstetter T. and Rühe J..
    Temperature and time resolved TIRF analysis of reusable DNA hydrogel chips.
    Anal. Chem., 2010, 82 (14), pp 6124–6131; DOI: 10.1021/ac1008578
  2. Rendl M., Bönisch A., Mader A., Schuh K., Prucker O., Brandstetter T. and Rühe J..
    A Simple One-Step Procedure for Immobilization of Biomolecules on Plastic Surfaces using Surface-Attached Polymer Networks.
    Langmuir, 2011, 27 (10), pp 6116–6123; DOI: 10.1021/la1050833
  3. Oswald Prucker, Thomas Brandstetter, and Jürgen Rühe
    Surface-attached hydrogel coatings via C,H-insertion crosslinking for biomedical and bioanalytical applications (Review)
    Biointerphases 2018, 13, 010801; DOI: 10.1116/1.4999786


  1. Brandstetter T., Böhmer S., Prucker O., Bissé E., zur Hausen A., Alt-Mörbe J. and Rühe, J. (2009).
    A Polymer-based DNA Biochip Platform for Human Papilloma Virus Genotyping.
    J Virol Methods. 2010 Jan;163(1):40-8. Epub 2009 Aug 5.; DOI: 10.1016/j.jviromet.2009.07.027
  2. Schenk T., Brandstetter T., zur Hausen A., Alt-Mörbe J., Huzly D. and Rühe J. (2009).
    Performance of a polymer-based DNA chip platform in detection and genotyping of human papillomavirus in clinical samples.
    J Clin Microbiol.; 47(5):1428-35.; DOI: 10.1128/JCM.02080-08
  3. Riehle U., Mader A., Brandstetter,T., Rühe J., zur Hausen A. and Stickeler E.
    Evaluation of NASBA in formalin-fixed and paraffin-embedded (FFPE) breast cancer tissues.
    J Clin Pathol 2010;63:1071-1076; DOI: 10.1136/jcp.2010.078766
  4. Mader A., Riehle U., Brandstetter T., Stickeler E., zur Hausen A. and Rühe J..
    Microarray-based Amplification and Detection of RNA by Nucleic Acid Sequence-Based Amplification (NASBA).
    Anal Bioanal Chem. 2010 Aug;397(8):3533-41. Epub 2010 Jul 2.; DOI: 10.1007/s00216-010-3892-4
  5. Riehle U., Mader A., Brandstetter T., Rühe J., zur Hausen A., Stickeler E.
    A Biochip for Simultaneous Gene Expressions-and SNP Analysis in Breast Cancer.
    Onkologie, 33, 142-142, 2010; DOI: 10.1055/s-0029-1225019
  6. Mader A., Riehle U., Brandstetter T., Stickeler E. and Ruehe J.. Universal nucleic acid sequence-based amplification for simultaneous amplification of messengerRNAs and microRNAs.
    Anal Chim Acta. 2012 Nov 19;754:1-7.; DOI: 10.1016/j.aca.2012.09.045
  7. C. Carstens, N. Hlawatsch, C. Gärtner, T. Brandstetter, J. Rühe, R. Elbracht.
    Lab-on-a-chip system for meningococcal diagnosis: automated sample processing of liquor or blood culture in microfluidic structures for detection of currently occuring Neisseria meningitidis strains
    CCCLM, 49(10)
  8. Nadine Hlawatsch; Richard Klemm; Cornelia Carstens; Thomas Brandstetter; Holger Becker; Rudi Elbracht; Claudia Gärtner.
    A lab-on-a-chip system for the development of complex assays using modular microfluidic components'''
    Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510D (February 9, 2012); DOI: 10.1117/12.910269


  1. Moschallski M., Baader J., Prucker O. and Rühe J..
    Printed Protein Microarrays on Unmodified Plastic Substrates.
    Anal Chim Acta. 2010 Jun 25;671(1-2):92-8. Epub 2010 May 13.; DOI: 10.1016/j.aca.2010.05.008
  2. Moschallski M., Evers A., Brandstetter T., Rühe J.
    Sensitivity of microarray based immunoassays using surface-attached hydrogels.
    Analytica Chimica Acta, 2013, 781, 72-79. DOI: 10.1016/j.aca.2013.04.013
  3. Yodmongkol S., Sutapun B., Praphanphoj V., Srikhirin T., Brandstetter T. and Rühe J.
    Fabrication of protein microarrays for alpha fetoprotein detection by using a rapid photo-immobilization process.
    Sensing and Bio-Sensing Research, Volume 7, March 2016, Pages 95–99. DOI: 10.1016/j.sbsr.2016.01.010


  1. Baader J., Klapproth H., Bednar S., Brandstetter T., Rühe J. and Freund I..
    Polysaccharide microarrays with a CMOS based signal detection unit.
    Biosensors and Bioelectronics, Volume 26, Issue 5, 15 January 2011, Pages 1839-1846, DOI: 10.1016/j.bios.2010.01.021
  2. Klapproth H., Bednar S., Baader J., Lehmann M., Freund I., Brandstetter T. and Rühe J.
    Development of a multi-analyte CMOS sensor for point-of-care testing.
    Sensing and Bio-Sensing Research, Volume 5, September 2015, Pages 117–122. DOI: 10.1016/j.sbsr.2015.08.004

Plug-based immunoassay:

  1. Rendl, M.; Brandstetter, T.; Rühe, J.
    Time-Resolved Analysis of Biological Reactions Based on Heterogeneous Assays in Liquid Plugs of Nanoliter Volume
    Analytical Chemistry, 2013, 85, 9469–9477.; DOI: 10.1021/ac401752j
  2. Rendl, M.; Brandstetter, T.; Rühe, J.
    Solid-Phase Extraction in Segmented Flow.
    Langmuir, 2014, 30, 12804–12811.; DOI: 10.1021/la502645z


  1. Frank Scherag, Thomas Brandstetter, and Jürgen Rühe
    Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings.
    Colloids and surfaces. B, Biointerfaces, 2014, 122, 576-82.; DOI: 10.1016/j.colsurfb.2014.07.035
  2. Frank D. Scherag, Robert Niestroj-Pahl, Solveigh Krusekopf, Klaus Lücke, Thomas Brandstetter, and Jürgen Rühe
    Highly Selective Capture Surfaces on Medical Wires for Fishing Tumor Cells in Whole Blood.
    Analytical Chemistry, 2017, 89, 1846-1854.; DOI: 10.1021/acs.analchem.6b04219
  3. Tamas Szell, Franz Dressler, Hanna Goelz, Benjamin Bluemel, Arkadiusz Miernik, Thomas Brandstetter, Frank D. Scherag, and Dominik Stefan Schoeb
    In vitro Effects of a Novel Coating Agent on Bacterial Biofilm Development on Ureteral Stents
    Journal of Endourology, 2018, November; DOI: 10.1089/end.2018.0616
  4. Frank D. Scherag, Andreas Mader, Marc Zinggeler, Nicole Birsner, Richard E. Kneusel, Thomas Brandstetter, and Jürgen Rühe
    Blocking-Free and Substrate-Independent Serological Microarray Immunoassays
    Biomacromolecules, 2018, November; DOI: 10.1021/acs.biomac.8b01334


  1. Marc Zinggeler, Patrick L. Fosso, Yan Hao, Thomas Brandstetter, and Jürgen Rühe
    Preparation of Linear Cryogel Arrays as a Microfluidic Platform for Immunochromatographic Assays
    Analytical Chemistry2017, 89, 5697–5701.; DOI: 10.1021/acs.analchem.7b01182
  2. Marc Zinggeler, Jan-Niklas Schönberg, Patrick L. Fosso, Thomas Brandstetter, and Jürgen Rühe
    Functional Cryogel Microstructures Prepared by Light-Induced Cross-Linking of a Photoreactive Copolymer.
    ACS Appl. Mater. Interfaces 2017 Apr 12;9(14):12165-12170.; DOI: 10.1021/acsami.7b01232
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